A comparison of two techniques for the determination of serum bone-specific alkaline phosphatase activity in dogs

Abstract

Bone-specific alkaline phosphatase (BALP) shows potential as a marker of bone formation in the dog. Recent studies have indicated that serum BALP may provide a useful, non-invasive indicator of skeletal health in dogs, and as a diagnostic and prognostic marker in the management of dogs with musculoskeletal or metabolic disorders. Two assay techniques (one based on wheatgerm lectin precipitation followed by a simple enzymatic reaction, the second on a specific enzyme-linked immunoassay) were used to measure serum levels of BALP in 35 dogs of different ages.As expected,BALP concentrations decreased with age. For the enzymatic assay, mean (±SD) serum concentrations ofBALP activities were 100·3 (±11·6) U/liter in dogs under 1 year of age, 25·3 (±6·8) U/L in dogs 1 to 2 years of age, 16·5 (±7·3) U/L in dogs 2 to 3 years of age, 14·3 (±5·6) U/L in dogs 3 to 7 years of age, and 12·3 (±4·8) U/L in dogs aged 8 years and older. Corresponding results from the immunoassay were 56·3 (±9·8) U/L, 10·7 (±4·5) U/L, 7·0 (±2·5) U/L, 6·7 (±3·6) U/L and 7·0 (±2·9) U/L. There was excellent correlation between the results from the two assay techniques (r = 0·96; P < 0·0001). The correlation between BALP and total ALP activities was poor (r = 0·20 for enzymatic BALP, r = 0·31 for immunoreactive BALP), indicating that total ALP should be considered unreliable as an indicator of BALP activity in canine serum. The immunoassay demonstrated acceptable (13 per cent) cross-reactivity with the liver isoform ofALP .The commercial immunoassay kit is simple and provides fast results. Although the wheatgerm lectin/enzymatic technique is preferred in situations where the activities of all three isoforms of ALP are required, the immunoassay should be considered whenever the activity of BALP is the focus of interest.