A Comparison of Three Methods of Extraction for the Determination of Polyphenols and Organic Acids in Tobacco by UPLC–MS–MS

Abstract

Matrix solid phase dispersion (MSPD), ultrasonic extraction followed by a solid phase extraction (USE–SPE) and reflux extraction (REFLUX) were studied for the analysis of polyphenols and organic acids in tobacco. The analysis was by ultra-high performance liquid chromatography tandem mass spectrometry (UPLC–MS–MS). The multi-mode support sorbent Zirconia/AA12S50 in MSPD is more suitable for the extraction of tobacco polyphenols than conventional silica or C18 silica. Although the matrix effect of USE–SPE is slightly stronger than MSPD and REFLUX for most target compounds, it gave higher extraction capacity, recoveries and sensitivity.