Abstract
Phase separated monolayers regularly utilize fluorescent probes as contrast agents to image two phase systems at the air-water interface. Langmuir monolayers have long been a model system for the lipid packing in individual leaflets. Imaging techniques are often used to study micron scale domain morphology as a simple binary system. However, fluorescence intensity likely offers further insights into monolayer packing and lipid environments. Monolayer fluorescence intensities containing DMPC with varying concentrations of Texas Red DHPE, 16:0 NBD PE, and 16:0-06:0 NBD PE have been measured while under compression. Experimental data is compared to idealized models of concentration vs. fluorescence intensity. Pressures-area isotherms will provide complementary information to the fluorescence intensity and packing density. The use of intensity data within monolayer studies offers the potential to offer new experimental approaches to traditional monolayer imaging studies.
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