Abstract
Aqueous saline extracts of fresh specimens of twelve species of marine algae, representative of the Chlorophyceae, Rhodophyceae and Phaeophyceae, have been analyzed by disc electrophoresis in polyacrylamide gels. The pattern of proteins in the green algae was the most complex with ∼12 bands, that in the reds less complex with ∼9 bands and least in the browns with ∼5 bands. Fractionation of the extracts with (NH 4) 2SO 4 indicated the probable presence of several globulins and chromoproteins, and two albumins, but such fractions were grossly heterogeneous. Fractionation of the extract from Rhodymenia palmata indicated the presence of allophycocyanin, R-phycocyanin and R-phycoerythrin mainly from spectrometric data. Trials with gradient gels and isoelectric focusing were less informative than by the conventional method of electrophoresis in 7·5 % gels. Comparison of six stains for algal proteins indicated Coomassie Brilliant Blue and Woolfast Blue as preferable.
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