Abstract
The Percoll self-forming gradient method has been applied for the preparation of plasma membranes from white adipose tissue and isolated adipocytes from mouse, rat, rabbit, ox and chicken. Characterization of the membranes produced by marker enzyme analysis confirmed the general applicability of the method. The polypeptide composition of the membranes was studied by polyacrylamide gel electrophoresis. The patterns seen with tissue and adipocytes of a single species source were compared and the Coomassie blue and periodic acid Schiff staining bands characteristic of each species were compared systematically. Additionally materials from lean (FA/-) and obese (fa/fa) Zucker rats were compared. The species-specific differences in polypeptide composition form a useful basis for the further development of methods whereby the adipocyte-differentiated phenotype may be detected in a wide range of systems.
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