Abstract
1. 1. Oxygen dissociation properties of red blood cell suspensions from Thunnus albacares, T. obesus, T. alalunga and Acanthocybium solandri are compared where the temperature and pH are varied independently. 2. 2. A change in the standard sigmoidal curve from of the dissociation curves was observed in low pH (⊃7·1)_treatment of each species except T. alalunga. This compares to a report on T. thynnus hemoglobin solution properties. 3. 3. Study of the blood pH of Scomber japonicus under anesthesia indicates that S. japonicus can survive a pH range from 6·8 to 7·4. Physiological values are assumed to be near but below pH 7·4. 4. 4. The effects of changes in temperature on half saturation of hemoglobin with oxygen are small compared with mammalian hemoglobin observations. The effect of temperature on curve form is marked, indicating an interaction between hemoglobins resulting in minimal shifts in P 50 as compared to other saturation levels. 5. 5. The effects of changes in pH are typical of those observed in mammalian systems.
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