Abstract
The lipids of the interscapular brown fat of bats, Myotis lucifugus, have been analyzed by silicic acid column chromatography and anion-exchange chromatography of the mild alkaline hydrolysis products of phosphatides. Brushite (CaHPO 4) chromatography has been introduced as an adjunct technique for the convenient separation of neutral from polar lipids and the removal of phosphatidylcholine from other phosphatides. Data from comparative studies of tissue obtained from male and female bats sacrificed during the hibernating and non-hibernating seasons are presented. The fatty acid composition of the cholesterol ester and triglyceride fraction is reported. The triglycerides, the major class of lipids, contain predominantly unsaturated fatty acids. Oleic acid is the principal individual fatty acid of this fraction. All brown fat preparations have high levels of cardiolipin presumably related to the rich content of mitochondria in brown adipose cells.
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More From: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
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