Abstract
AbstractTwo rapid staining procedures using 8‐anilino‐1‐naphthalene sulphonate (ANS) and Nitro Blue Tetrazolium (NBT) were compared with a Coomassie Brilliant Blue R‐250 staining technique. The methods were applied to protein separations using both one‐ (1‐D) and two‐dimensional (2‐D) polyacrylamide gel electrophoresis. The ANS procedure worked well for both 1‐D and 2‐D gels. The NBT technique, although more rapid, gave satisfactory results only for 2‐D gels. The ANS and NBT staining methods were both compatible with Western blotting so that they could be used for visualisation of the separation profile prior to blot transfer to nitrocellulose.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
