Abstract
Normal callus culture and habituated embryogenic suspensor mass (ESM) of silver fir (Abies alba Mill.) were compared enzymatically using three enzyme systems. Significant reduction of peroxidase activity, more intensive glutamate dehydrogenase activity and higher number of isoenzymes of non‐specific esterase were found in habituated ESM as compared with the normal calli. Defense reactions of normal callus culture and habituated ESM were tested by a simple method of dual cultures. Habituated ESM showed strengthened defense reactions against tester Phaeolus schweinitzii. Reasons of this phenomenon are discussed.
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