A comparison of methods for the detection of Ascochyta rabiei in chickpea seeds

Francisco José Sautua,Santiago Agustín Casey,Marcelo Aníbal Carmona,Raúl Lorenzo Zapata,María Mercedes Scandiani

doi:10.1590/0100-5405/185396

Francisco José Sautua, Santiago Agustín Casey + Show 3 more

Open Access

https://doi.org/10.1590/0100-5405/185396

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Abstract

ABSTRACT Seed health is one of the most important factors affecting the quality of chickpea (Cicer arietinum) seeds. The present study aimed to compare and identify the best incubation methods for detecting Ascochyta rabiei associated with chickpea seeds. Four protocols were compared for their sensitivity in detecting A. rabiei: T1) Incubation on paper substrate or filter paper method (blotter test) without surface disinfection, T2) Blotter test through the water restriction technique, T3) PDA plate test, and T4) MEA plate test. Four independent chickpea seed lots, naturally infected with A. rabiei, were sampled from Córdoba Province and other four were sampled from Buenos Aires Province, Argentina. Each treatment was applied to a total of 400 seeds from each locality for the methods to be comparable. T2 and T3 were statistically more sensitive in detecting A. rabiei-infected seeds from Córdoba. Only these two treatments were repeated for seeds from Buenos Aires Province, and T3 proved to be more sensitive; thus, it is recommended for routine sanitary analysis of chickpea seeds.

Highlights

A saúde das sementes é um dos fatores mais importantes que afetam a qualidade das sementes de grão-de-bico (Cicer arietinum)
The four treatments consisted in: T1) Standard blotter test (SBT): seeds without surface disinfection were plated on sterilized water-soaked filter papers in plastic trays and were incubated at 21°C+/
T3) PDA plate test: seeds were surface-disinfected with 0.1% sodium hypochlorite (NaOCl) solution for 1-2 minutes; they were rinsed with demineralized sterile water for one minute and allowed to dry on sterile filter paper

Summary

Introduction

A saúde das sementes é um dos fatores mais importantes que afetam a qualidade das sementes de grão-de-bico (Cicer arietinum). Quatro lotes de sementes de grãode-bico independentes, naturalmente infectados com A. rabiei, foram amostrados na província de Córdoba e quatro na província de Buenos Aires, Argentina. The objective of the present study was to compare different incubation methods for the detection of A. rabiei associated with chickpea seeds.

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