Abstract
The radioimmunoassay for DNA-antibodies in systemic lupus erythematosus is assessed with DNA labelled with 125I via chemical iodination on one hand ( 125I-DNA), and with DNA containing [ 125I]iododeoxyuridine via biological incorporation on the other ([ 125IUdR]DNA). The results show that chemical iodination labels protein impurities in the DNA and reduces the difference in binding of normal vs lupus sera. 125IUdR-DNA is a superior product since no label is introduced in proteins. The binding of 125I-DNA by normal sera ranges from 5 to 20% and from 40 to 70% lupus sera. The same sera yield values of 0–3% for normal and 92–98% for lupus with 125IUdR-DNA. The latter allows a sharper discrimination between normal and slightly elevated values, as in patients under treatment.
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