Abstract
Three image analysis software, Photoshop, Sorbfil TLC Videodensitometer software and Scion Image,were used for quantitative evaluation of TLC images of ceftriaxone sodium (CFX). Regression plots anddetection sensitivity of quantification from each TLC-image analysis were determined and compared toTLC-densitometry. TLC-image analysis method using Scion Image and Sorbfil TLC Videodensitometersoftware and TLC-densitometry showed the polynomial regression data with good relationship (R2 > 0.99)over the concentration range of 1-8 mg/spot whereas the analysis of TLC images with Photoshop showedgood polynomial regression plots (R2 > 0.99) at higher concentration range, 4-10 mg/spot. For detectionsensitivity, LOD and LOQ determined from TLC-image analysis using Sorbfil TLC Videodensitometer werecomparable to the values from TLC-densitometry. Scion Image and Photoshop was found to be less sensitive.The use of Sorbfil TLC Videodensitometer software for TLC image analysis could be further applied forrapid determination of CFX in bulk drug and dosage forms. Key Words: TLC; Image analysis software; Ceftriaxone sodium
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