Abstract
Abstract The O'Farrell method of high resolution two-dimensional electrophoresis (1) is widely used for analysis of complex protein mixtures (2, 3). Among the described modifications to this technique are changes in the sodium dodecyl sulfate (SDS) equilibration step associated with the transfer of first dimension isoelectric focusing (IEF) gels to second dimension polyacrylamide electrophoresis gels (4–7). Wright (8) has recently recommended SDS equilibration in aqueous 55% methanol for improved resolution. Since we have encountered problems applying this approach to our particular two-dimensional gel system, a simpler, rapid, and more convenient method based on IEF gel shrinkage in 100% glycerol is now recommended.
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