Abstract
AbstractA chemiluminescence immunoassay (CLIA) for methyltestosterone is compared with a radioimmunoassay (RIA) employing the same antiserum, raised against methyltestosterone‐3‐CMO‐BSA and using N‐(4‐aminobutyl)‐N‐ethylisoluminol conjugate of MT and [1,2‐H3]methyltestosterone as tracers.Muscle tissue from slaughtered animals was selected as the matrix. After enzymatic digestion and diethylether extraction only a limited sample clean‐up on Lipidex‐5000 and on Bond Elut C18 was required. Both methods had similar limits of detection, sensitivities, limits of quantification and speed of analysis (working‐load and availability of results).
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