A comparison of biomarker responses in the earthworm Aporrectodea caliginosa to the organophosphorus insecticides diazinon and chlorpyrifos.

Abstract

Three biomarkers in Aporrectodea caliginosa Savigny were evaluated for their ability to detect exposure to organophosphorus insecticides, and these physiological responses were related to effects on growth and life-table parameters. Adult and juvenile earthworms were exposed to a laboratory-simulated field rate (low concentration) and a higher sublethal concentration of diazinon and chlorpyrifos. After a four-week exposure, juveniles were evaluated for cholinesterase activity, glutathione S-transferase activity, and growth, and adults were evaluated for the lysosomal neutral red retention assay (NRRA) and growth. Cholinesterase activity and the NRRA were more sensitive than growth in each age group for detecting exposure to the pesticides. Life-table parameters were evaluated in earthworms exposed as juveniles and as adults. Maturation was less sensitive to pesticides than was cocoon production. Growth and cocoon production in earthworms exposed as juveniles appeared to be more sensitive to organophosphorus insecticides than earthworms exposed as adults. Life-table responses in juveniles may, therefore, be more predictive of long-term impacts of organophosphorus insecticide applications on populations than responses in adults. Biomarker responses occurred at similar or lower concentrations than those causing an adverse effect on cocoon production and cocoon viability, indicating their usefulness in risk assessment for predicting ecologically relevant assessment end points.