Abstract
Abstract The benzo(a)pyrene-4,5-epoxide (BP-4,5-epoxide) hydrase activities of intact hamster hepatocytes and embryo cells, and homogenates of these cells as well as of adult liver are compared. The product of the epoxide hydrase (EH) reaction, trans-4,5-dihydro-4,5-dihydroxybenzo(a)pyrene (BP-4,5-diol), was isolated by high-pressure liquid chromatography (HPLC) with a Waters Bondapak C18/Corasil column and acetonitrile-water as the mobile phase. Using this procedure to determine BP-4,5-epoxide hydrase activity in intact cells, it was found that 266 nmoles of BP-4,5-diol/106 cells were produced by hepatocytes while no diol formation was detected with embryo cells. EH activity in the intact hepatocytes was 8-fold greater than in hepatocyte homogenates.
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