A comparison in individual murine tumors of techniques for measuring oxygen levels

Abstract

Purpose: To investigate the relationship between different techniques for measuring oxygen levels in a murine tumor model. Methods and Materials: Using the murine fibrosarcoma line KHT-C, five techniques of measuring oxygen levels—the Eppendorf pO 2 Histograph, EF5 binding, the comet assay, a paired survival assay, and an in vivo growth delay assay—were assessed. In these experiments, three or more techniques were applied in different combinations to measure the oxygen levels in individual tumors. Results: Statistically significant correlations were observed between the hypoxic proportions calculated from the paired survival assay with those from EF5 binding. The comet assay was found to have a statistically significant correlation with the paired survival analysis and the growth delay analysis. No statistically significant correlation was found between the Eppendorf pO 2 Histograph measurements and those from the other techniques, although there were weak correlations with the paired survival assay and EF5 binding. For technical reasons, a comparison was not made between EF5 binding and the growth delay assay. Conclusions: The correlations found between EF5 binding and the comet assay with the radiobiological assays suggest that these techniques have potential for predicting outcome following radiation treatment. The lack of correlation seen between the pO 2 Histograph data and the radiobiological assays is in contrast to results from early clinical trials.