Abstract
In the present study, two commercial assays for the redox status are compared and evaluated, the SH groups in proteins (SHp) and the total thiol levels (TTL). Both assays are colorimetric endpoint reactions and have been adapted for use on an automatic clinical analyzer. Both assays performed well by giving comparable results in plasma samples. A good correlation with a correlation coefficient (R2) of 0.889 was found between the two assays. However, for some pathological specimens, especially highly lipemic samples, the SHp assay performed better than the TTL assay. When plasma samples with a high lipemic index were omitted, the correlation between the two assays was even better with an R2 of 0.942. In conclusion, both assays for the redox status performed well, but the SHp assay can also be used in high lipemic samples.
Highlights
The redox status is an important tool in determining the physiological state of the body, especially for the elderly and with hypertension and heart failure [1,2,3,4]
A reflection of the redox status in the circulation can be measured by the level of free thiol groups in proteins in serum or plasma samples
The most used assay is based on the reaction of 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), with thiol groups which can be performed with home-made materials [5,6]
Summary
The redox status is an important tool in determining the physiological state of the body, especially for the elderly and with hypertension and heart failure [1,2,3,4]. There are several assays described in the literature for the evaluation of the redox status in serum or plasma In these simple assays, a reaction of thiol groups of proteins takes place with a chromogen. For quality control during large-scale studies, commercially available assays are preferred due to the long-term delivery and stability of the assay components and the availability of quality control materials This test can be performed either by high performance liquid chromatographic methods [7,8,9] or in micro plate format [10] and was programmed and applied to clinical analyzers [11,12,13,14]. The use of auto-analyzers allows measurement of large sets of samples in epidemiological studies under the required quality during longer periods. Another advantage is the possibility to combine the assay with the measurement of other biomarkers in the same small volume of sample [15]
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