Abstract
The World Health Organization (WHO) and many studies considered the infertility as a disease and so many couples complaining from unsuccessful assisted reproductive technologies procedures to overcome their problem. One of the reasons of this dilemma is the sperm preparation method when no optimum result obtained even by using any of media found globally. However Global sperm washing®, and FertiCult flushingTM media were proved their capability to obtain good results of certain sperm function parameters. Nevertheless, the studies that compare between these media were rare. Therefore, this study aimed to compare between Global sperm washing medium®, FertiCult flushing TM media that used for sperm washing before using the partner sperm for ART procedure. After detecting asthenozoospermia in sixty semen samples, they were divided into two groups according to medium used for sperm activation in vitro Global sperm washing medium ® (n=31) and FertiCult flushing mediumTM (n=29) groups.The semen analysis was done after 3-5 days of abstinence as recommended by the manual of WHO (1999). Certain sperm function parameters were recorded. Semen fluid samples were treated with sperm activation media (Global sperm washing medium and FertiCult flushing medium TM) by using direct swim-up technique for in vitro sperm activation test. A significant (P<0.05) improvement was noticed between the two media regarding active sperm motility grades A and B when using FertiCult flushing mediumTM compared to Global sperm washing medium®. Whereas no significant (P>0.05) differences were detected between the two media regarding sperm motility grades C and D. There was no significant (P>0.05) differences in morphologically normal sperm following in vitro activation by using the two media. It is concluded that FertiCult flushing mediumTM was better than Global sperm washing medium® in improving active sperm motility of asthenozoospermic men which can be utilized in future for successful of assisted reproduction.
Highlights
A Comparison Between the Effects of Global Sperm Washing® and FertiCult Flushing TM Media on Certain Sperm Function Parameters of Asthenozoospermic Men
FertiCult flushing TM ووسط, Global sperm washing medium ® وسط, وهن النطف:الكلمات الدالة Introduction Many people assume that infertility is a" man or woman's "problem, but many cases of infertility are a result of a man factor too
The successful rate and pregnancy rate of artificial insemination in husband (AIH) varies considerably, and researchers do their best to stand on the causes of low success rate of AIH to find the best procedures that improve male fertility, which through the semen must be present in sperm motile, normal morphology, and be able to penetrate an ovum [3]
Summary
Sperm preparation and washing are usually performed by different media such as Sage®, In Vitro life®, Global®, FertiCultTM, MediCult® and others to wash any semen sample for intrauterine insemination (IUI) and other assisted reproductive technologies (ARTs) procedures. Sperm washing achieves two very important goals It removes most of the seminal plasma from the semen that would otherwise not react well if directly inseminated into the uterus. It concentrates the sperm density into a small volume that is suitable for any assisted reproduction technologies.
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