A Comparative Study on the Protective Role of Obestatin, Quercetin and their Combination on the Injured Rat Gastric Mucosa Induced by Stress

Abstract

AbstractBackground: Modern life contains a lot of nervous stresses which considered as the origin of many diseases.Aim of Study: Comparison between the protective effect of obestatin, quercetin and their combination on the stress-induced gastric ulcer in rats.Material and Methods: Sixty healthy adult Wistar male albino rats weighed 180-220g were used. Ten rats were con-sidered as normal control (Group I). Stress-induced gastric ulcers were produced in the other 50 rats. These rats were divided into 5 groups: Group II (with no medication), Group III (pretreated with misoprostol), Group IV (pretreated with obestatin), Group V (pretreated with quercetin) and Group VI (pretreated with both obestatin and quercetin). All medi-cations were used for 2 weeks before induction of ulcer.Results: In all groups the following parameters were measured; TBARS, antioxidant enzymes activities, myeloper-oxidase activity in gastric tissue, ulcer index, and plasma tumor necrosis factor-a. Comparing the results of Groups IV and V to that of Group II showed that, the use of obestatin or quercetin separately can ameliorate the condition to some extent with variable superiority. Also, the combined pretreat-ment of these rats with obestatin and quercetin produced more significant improvement; approaching normal; of all the tested parameters than the use of each drug separately. Moreover, the results in Group VI were far superior over the use of the standard drug misoprostol in Group III.Conclusion: The combined use of obestatin and quercetin as a prophylactic measure against the development of stress-induced gastric ulcer is more effective than the use of each drug separately.