Abstract
The metabolism of 4-androstene-3, 17-dione (androstenedione) by liver microsomes from hagfish, Myxine glutinosa, dogfish, Squalus acanthias, and rainbow trout, Salmo gairdnerii, was studied. The following reduced metabolites were formed in the hagfish: testosterone, 3α-hydroxy-5α-androstan-17-one and 5α-androstane-3, 17-dione. In addition, 3α-hydroxy-4-androsten-17-one was formed by liver microsomes from dogfish and rainbow trout. While the patterns of reduced metabolites were very similar, there was a marked variation in the patterns of hydroxylated metabolites produced by the different species. Liver microsomes from hagfish gave rise to 6β-hydroxytestosterone and 6β-hydroxyandrostenedione. In addition to 6β-hydroxylation, 6α- and 16β-hydroxylations were observed in liver microsomes from dogfish. Liver microsomes from rainbow trout performed all the hydroxylations mentioned and were also capable of 1α-hydroxylation. The formation of hydroxylated metabolites was inhibited by CO indicating the participation of cytochrome P-450-dependent enzymes.
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