Abstract
Aim: To evaluate the ability of red and white hibiscus (H) in improving the epithelial barrier in indomethacin-induced duodenal and colonic inflammation, compared with prebiotic and to investigate whether the red and/or white hibiscus can be used as natural prebiotic like agents. Methods: Histological, histomorphometric, histochemical demonstration for goblets cells and mucin intensity. Immunohistochemical demonstration of COX-2, and tight junctions (Claudin-1 and E-cadherin) were examined. Duodenal and colonic TNF-α, IL-6, IL-10, PGE2, MPO, MDA, TAC, besides, serum CRP were evaluated. Results: Indomethacin induced inflammation and ulceration in the duodenum and colon, with significant depletion in goblet cell count and mucin intensity. COX-2 was increased while claudin-1 and E-cadherin were significantly diminished. Tissue TNF-α, IL-6, MPO, MDA, and serum CRP were elevated significantly by indomethacin, while IL-10, PGE2 and TAC were reduced. Co-treatment with red H, white H, or prebiotics plus indomethacin improved significantly duodenal and colonic histoarchitecture, tissue contents of PAS cells, mucin, claudin-1 and E-cadherin, while COX-2 reduced. TNF-α, IL-6, MPO, MDA, and serum CRP were decreased significantly, while IL-10 , PGE2 and TAC were restored. Conclusion: The current research introduces red and white hibiscus as prebiotics-like agents because of their anti-inflammatory, antioxidant, or tight junctions modulating activities.
Highlights
Inflammatory bowel disease (IBD) is the term used to describe various conditions that induce chronic inflammation of the small and/or large intestine (Dodda et al, 2014)
The current results revealed that indomethacin-induced significant decrease in BWG%, feed-intake, Colon/ body weight ratio, intestino-somatic index (ISI) as well as histopathological and morphometric alterations in both duodenum and colon when compared with control rats
Concerning histochemical demonstrations, indomethacin resulted in apparent depletion in the number of goblet cells and mucin staining intensity in the duodenum and colon when compared with control group
Summary
Inflammatory bowel disease (IBD) is the term used to describe various conditions that induce chronic inflammation of the small and/or large intestine (Dodda et al, 2014). IBD causes significant gastrointestinal symptoms as diarrhea, abdominal pain, bleeding, anaemia and weight loss, and is considered as the major reason for many human cancers, including colorectal cancer (CRC) (Chougule et al, 2018; and Rajendiran et al, 2018). Formalin, indomethacin, and carrageenan could be used as inducers of IBD experimentally (Chougule et al, 2018). Aberrations and disruption in the epithelial barrier are regarded as the major event in pathogenesis of IBD (Goto et al, 2015). The gastrointestinal epithelial barrier is the first line of defense that designed as a continuous
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