Abstract
A Comparative Study on Staining Techniques for Vaginal Exfoliative Cytology of Rat
Highlights
IntroductionSpontaneous ovulators with regular and successive estrous cycles that may vary with age and species
Female rodents are polyestrous, spontaneous ovulators with regular and successive estrous cycles that may vary with age and species
Predominant cell type observed in this study during different stage of estrous cycle viz, proestrus with nucleated epithelial cells, estrus cornified squamous epithelial cells, metestrus shows equal proportion of leukocyte, cornified cells and the diestrus smear with predominance of leukocyte as described by [3,8]
Summary
Spontaneous ovulators with regular and successive estrous cycles that may vary with age and species. The short length of the estrous cycle of rats makes them ideal for investigation of changes occurring during the reproductive cycle [2].The orchestra of various hormones such as estradiol, progesterone, luteinizing hormone and follicle stimulating hormone which is a reflection of the hypothalamic-pituitary-ovarian axis functional status influence the cyclicity of the estrous cycle. Any changes in these hormones normal wave pattern will reflect on the vaginal cell pattern which can be identified by observing the daily vaginal exfoliative cytology. Evaluation of vaginal cytology under microscope has long been used to record the stages of estrous cycle in the laboratory animals
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