A comparative study on innate immunity parameters in the epidermal mucus of Indian major carps

Abstract

The innate immune system, particularly the external body surface, plays a frontier role in protecting fish from relevant infections. The present study is aimed at understanding and comparing the mucosal immunity of Indian major carp, that is, Labeo rohita, Catla catla and Cirrhinus mrigala, by evaluating different immune parameters such as protein content, lysozyme, myeloperoxidase, proteases and alkaline phosphatase activity in the skin mucus. The protein content of mucus sample of these species was compared, and the highest protein content was found in C. catla among the three Indian major carp species. The levels of proteases (40 ± 0.211 units/ml) and myeloperoxidase OD450 nm (1.525 ± 0.108) were found to be highest in the skin mucus of C. catla. However, lysozyme levels were highest in the skin mucus of L. rohita (10.95 ± 0.330 μg/ml), and C. mrigala had the highest alkaline phosphatase activity (30.74111 ± 0.680 U/l). Besides the enzyme activities, the epidermal mucus samples of three Indian major carp species were also tested and compared for the antibacterial activity against seven bacterial strains. Skin mucus of C. catla showed highest antibacterial activity among the three Indian major carps against all the seven bacterial strain, except that Micrococcus lysodeikticus and Vibrio cholerae, however, showed highest activity against mucus of C. mrigala. Also, the epidermal mucus from all the three species successfully agglutinated three freshwater pathogens, viz. Aeromonas hydrophila, Pseudomonas fluorescens and Edwardsiella tarda, with agglutination titres being the highest for Labeo rohita for all the pathogens. The epidermal mucus samples from the Indian major carp species showed haemagglutination activity and successfully lysed human, chicken and goat RBCs showing highest activity in C. catla. These results provide information for a better understanding of the role of epidermal mucus and its components in the innate immune system of Indian major carps.