Abstract
Purpose: To evaluate the microbial load on impressions and the efficacy of various disinfectants on reducing microorganisms from the impression surface after disinfection. Materials and Methods: A total of 50 dentulous impressions were made for dentate patients each of age 20 to 50 years using Alginate and Polyvinyl siloxane. Impression compound and zinc oxide eugenol were used for edentulous patients each of age 50 to 70 years, total of 50 edentulous impressions were made. Samples were collected with the help of an 8mm diameter sterile cork borer from each individual impression. Alginate, ZOE and impression compound had 100 samples each. Polyvinyl siloxane had 125 samples. Out of 100 samples the first group of the sample was assigned as control (n=25), the second group of samples (n=25) were immersed in 2% Glutaraldehyde, the third group of samples (n=25) were sprayed with Dimenol, the fourth group of samples (n=25) were placed in the ultraviolet light chamber. For polyvinyl siloxane, an additional fifth sample group (n=25) was subjected to microwave radiation of 650W. The viability of microorganisms that can persist after rinsing and disinfecting the impression surface was tested by inoculating in nutrient media. The colony forming units were counted and compared with the control group. The data were documented and statistically analyzed. Results: There was a significant difference in the count among the control group and the disinfectant groups. Microbial load found on control groups was taken as 100%. Alginate samples which were disinfected with 2% Glutaraldehyde, Dimenol spray and UV light showed microbial load 31%, 46%, 44% respectively, Polyvinylsiloxane samples showed microbial load 35%, 53%, 48% and 0% with microwave radiation respectively. Impression compound samples showed 32%, 49%, 49% respectively & Zinc oxide eugenol samples with 2% Gluteraldehyde, dimenol and UV showed a load of 39%, 51%, 51% respectively. Conclusion: 2% Glutaraldehyde showed hig
Highlights
Oral microflora is inhabited by numerous microorganisms
The purpose of this article is to evaluate the microbial load on impression and the efficacy of various disinfectants on the reduction of microorganisms from the impression surface after disinfection
A significant reduction in the microbial growth on different impression material surfaces treated with disinfectants compared to control group was observed
Summary
Oral microflora is inhabited by numerous microorganisms. Dental impressions and casts made from contaminated saliva and blood pose serious risk to dental personnel. Sterilization is the process by which all forms of microorganisms such as viruses, bacteria, fungi, and spores are destroyed.[1] Disinfection is generally a less lethal process when compared to sterilization. It virtually eliminates all recognized pathogenic microorganisms, but not necessarily all microbial forms (bacterial endospores), on inanimate objects.[2] Disinfectants used in dental settings must be registered as a hospital disinfectant by Environmental Protection Agency.[1] Surface disinfection will inactivate the infectious agents and reduce the spread of infection to dental personnel from contaminated impressions. The purpose of this article is to evaluate the microbial load on impression and the efficacy of various disinfectants on the reduction of microorganisms from the impression surface after disinfection
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