A comparative study of various light source influences on the plants regenerative potential using Populus berolinensis root explants as an example

Abstract

The paper presents the results of a comparative study of the effects of three light source types on micropropagation efficiency of Berlin poplar (Populus berolinensis). As a model explant type for regeneration root segments were used. Three types of light sources were compared: fluorescent cool white light lamps LED-based purple (red+blue) light phyto lamps and LED-based neutral white light phyto lamps. The poplar regeneration was more effective under fluorescent lamp illumination treatment then at both types of LED phyto lamps. Among two studied LED light sources, the best result was obtained for white light lamps. The quantity and size of regenerants obtained under LED white light lamps illumination were comparable with those got under fluorescence lamps. The least regeneration efficiency was observed under the purple light LED lamps illumination with a limited spectrum range of emitted light. Nevertheless, in case of discontinuation of fluorescent lamps due to the policy of efficient energy consumption and environment protection optimized LED phyto lamps illumination has prospects in micropropagation of plants.