Abstract
A molecular cytogenetic survey of Cucumis sativus L. revealed a chromosomal polymorphism among 2 European pickling cultivars of ‘Borszczagowski’ and ‘Monastyrski’, and one Japanese cultivar of ‘Zibai’ by fluorescent banding method with chromomycin A3 (CMA) and 4′,6-diamidino-2-phenylindole (DAPI), and fluorescence in situ hybridization (FISH) with the probes of 5S, 45S rDNA and telomere repeat sequences. Heterochromatic banding polymorphism was found in some chromosomes with CMA and DAPI staining, whereas no chromosomal polymorphism was found in FISH. Thus, the FISH allowed us to identify all of homologous chromosomes in each cucumber cultivars, while CMA and DAPI banding showed an easy detection of the polymorphism in cucumber chromosomes, even in close related cultivars between European pickling cultivars.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
