Abstract
Dietary 18 and 20-carbon fatty acids of the n-6 and the n-3 families are metabolized to 22:5, n-6 and 22:6, n-3 by a sequence of specific desaturases and chain elongation via 24-carbon intermediates. This pathway is regulated so that more 22:6, n-3 than 22:5, n-6 is found in the tissues. Rat testis is an exception since 22:5, n-6 is present in large proportions in this organ. Therefore rat testis appears to be interesting for studies of the detailed synthesis of 22:5, n-6 compared with that of 22:6, n-3. By using fresh preparations of rat testicular cells from 19-day-old rats enriched in Sertoli cells, we compared the metabolism of 1- 14C-labelled n-3, n-6 and n-9 fatty acids. The testicular cells actively synthesized 22:6, n-3 and 22:5, n-6, but not 22:4, n-9 from the 18 and 20-carbon precursors. Of 200 mol 14C-labelled C 18 and C 20 fatty acids added initially, approximately 20-40 mol were found as 24-carbon intermediates after 24 h of incubation. This indicates that the balanced capacity of elongation, desaturation and chain shortening favours the accumulation of 24-carbon intermediates in these cells. One exception was [1- 14C]20:3, n-9 which was efficiently elongated to 22:3, n-9 but not to C 24 fatty acids. Our data suggests that the poor elongation of n-9 fatty acids from C 22 − to C 24 may be an important hindrance in the synthesis of 22:4, n-9. The efficient synthesis of 22:5, n-6 may also partly explain why this is the major 22-carbon fatty acid in rat testis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.