Abstract
1. 1. Five esterase isozymes were found from soluble extracts of Anopheles punctipennis. 2. 2. Each of the isozymes exhibited a differential specificity depending on the type of inhibitor used. 3. 3. The A and B loci were characterized as specific cholinesterases based on the response to treatment with a variety of organophosphates and carbamates. 4. 4. The other loci did not respond as uniformly to the various pesticides used in this study, but were characterized as either carboxylesterases or aromatic esterases for discussion purposes. 5. 5. The importance of having pure isozyme preparations is considered in conjunction with additional knowledge of the kinetic and stereospecific properties of the inhibitors. 6. 6. The techniques described in this work showed that mosquito isozymes may be clearly separated with a high degree of resolution.
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