A Comparative Study of Survival, Metabolism, Immune Indicators, and Proteomics, in Five Batches of Japanese Scallop Mizuhopecten yessoensis under Short-Term High Temperature Stress

Abstract

Five batches of the Japanese scallop Mizuhopecten pyessoensis were tested for survival rate, oxygen consumption, catalase (CAT) and superoxide dismutase (SOD) activities, total antioxidant capacities (T-AOC) contents, and proteomics under short-term high temperature conditions. The five batches, (W1, W2, W3, W4, W5) selected from the established 21 ‘ivory white’ M. yessoensis batches, had higher survival rates than the other batches after one year of culture. Initial rearing water temperature of 15°C was increased by 1°C per day with a cooling and heating system. The temperature was raised until over 50% of the scallops from 3 batches died. This occurred at 30°C. The higher than normal culture temperature conditions showed significant or highly significant differences in the responses of some of the batches. Some showed significantly higher survival rates and significantly different rates of oxygen consumption. CAT activity, SOD activity and T-AOC content was similar in the five batches, and all three indices were significantly lower in W3 and W5 than in the other batches (P<0.01). Expression patterns of MDA content were opposite to those of CAT activity, SOD activity and T-AOC content. Protein profiles of all five batches were similar; the sizes of the predominant bands ranged from 20-110 kDa. We identified twenty-eight proteins with high scores in the database. These included heat shock proteins (HSPs), glucose-regulated protein 94, and arginine kinase.