Abstract
An inoculation technique, in which cultures of Leptosphaerulina briosiana (Poll.) Graham & Luttrell grown on filter paper soaked in 20% mixed vegetable juice, V-8 brand, are suspended over alfalfa plants and allowed to discharge spores onto them, was developed and compared with previously described inoculation methods. The new method was found to be superior and the inoculum, in contrast to that of the similar V-8 agar method, was more easily prepared and applied, could be watered to prevent drying our and could be stored in a frozen state between inoculations. Less than 5% of the seedlings and mature plants of four species of Medicago inoculated by this method appeared to be resistant.
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