Abstract

BackgroundVariation in the genomes of single-stranded RNA viruses affects their infectivity and pathogenicity in two ways. First, viral genome sequence variations lead to changes in viral protein sequences and activities. Second, viral genome sequence variation produces diversity at the level of nucleotide composition and diversity in the interactions between viral RNAs and host toll-like receptors (TLRs). A viral genome-typing method based on this type of diversity has not yet been established.Methodology/Principal FindingsIn this study, we propose a novel genomic trait called the “TLR stimulatory trimer composition” (TSTC) and two quantitative indicators, Score S and Score N, named “TLR stimulatory scores” (TSS). Using the complete genome sequences of 10,994 influenza A viruses (IAV) and 251 influenza B viruses, we show that TSTC analysis reveals the diversity of Score S and Score N among the IAVs isolated from various hosts. In addition, we show that low values of Score S are correlated with high pathogenicity and pandemic potential in IAVs. Finally, we use Score S and Score N to construct a logistic regression model to recognize IAV strains that are highly pathogenic or have high pandemic potential.Conclusions/Significance Results from the TSTC analysis indicate that there are large differences between human and avian IAV genomes (except for segment 3), as illustrated by Score S. Moreover, segments 1, 2, 3 and 4 may be major determinants of the stimulatory activity exerted on human TLRs 7 and 8. We also find that a low Score S value is associated with high pathogenicity and pandemic potential in IAV. The π value from the TSS-derived logistic regression model is useful for recognizing emerging IAVs that have high pathogenicity and pandemic potential.

Highlights

  • In single-stranded RNA viruses, genome sequence diversity affects infectiousness and pathogenicity in two ways

  • We develop a computational method to evaluate the ability of single-stranded RNA virus genomes to stimulate toll-like receptors (TLRs) 7/8 based on their nucleotide composition

  • We focus on stimulatory activity toward human TLRs 7/8 because all of the TLRs 7/8 stimulatory oligoribonucleotide (ORN) sequences we examined were collected from literatures that used human cells as experimental models

Read more

Summary

Introduction

In single-stranded RNA viruses, genome sequence diversity affects infectiousness and pathogenicity in two ways. Diversity in viral genome sequences leads to alternations of viral protein sequences and, changes in viral protein activity that may affect replication, transmission or antigenicity (interactions with the host’s adaptive immunity). Diversity in viral genome sequences results in differences in nucleotide composition that may affect the stimulatory activity that viral RNAs exert on host TLRs. Genome sequence diversity may provide a way for single-stranded RNA viruses to evade host innate immunity. Viral genome sequence variation produces diversity at the level of nucleotide composition and diversity in the interactions between viral RNAs and host toll-like receptors (TLRs). A viral genome-typing method based on this type of diversity has not yet been established

Methods
Results
Conclusion

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.