A Comparative Study of High Level laser and Low Level laser and Temperature on the Erythrocytes Sedimentation Rate Values in Healthy People : In Vitro Study

Abstract

Background :The effects of laser irradiation with 632.8 and 2100 nm on Erythrocyte Sedimentation Rate (ESR) in blood samples from healthy peopleblood were comparatively studied in vitro and study the effect of environmental temperature. Objective : This study was designed to show the effect of He-Ne laser , HO-YAG laser and effect of environmental temperature on Erythrocyte Sedimentation Rate (ESR) . Subject & Methods : The study of case – control cross sectional design included 40healthy persons (male and female) with age group (20-45 year) , Workplace in the laboratory of physiology and medical physicsthe time between April 2016 to July 2016. He-Ne laser  (LJL 40-HA, Shanghai Research Institute of Laser Technology) wave length of 632.8ηm was used for irradiation with 5mm diameter beam spot on blood samples, with power density  30mw/cm2 and HO-YAG laser irradiation wave length 2100nm          ( Karl Storz – Endoskope , Germany ),output power is ( 0.5J) .The irradiation times were (5min ) , The samples of blood were obtained from 40 contributors and each sample was separated into three samples for irradiation and control .All statistical analysis was performed withstatistical package graph Pad software For those with significant difference.                                                                                                                    Results : The results showed that there were significant differences in the values of sedimentation rates for both class (15 -45 ) 0c degree heat compared with the values of sedimentation 15 0c degree heat temperature for irradiation and control It shows the effect of temperature factor on the red blood cells sedimentation rate in blood samples from healthy people and see the effect of the degree of laboratory heat due to lack of efficient heating and cooling devices in the summer and winter on the sedimentation rates of values to be taken into consideration with the results .We observe irradiation 632.8 nm laser decreases ESR while irradiation at 2100 nm increases ESR with p < 0.01. Conclusion:The change of temperature (high and low) has a great effect on  precipitation of red blood cells in health people and thus must be taken into consideration and this effect also observed with the use of632.8 and 2100 nm on Erythrocyte Sedimentation Rate (ESR) in blood samples from healthy individuate  blood were comparatively studied in vitro at a temperature of 15 °C.We observe irradiation 632.8 nm laser decreases ESR while increasing irradiation at 2100 nm with p < 0.01.