Abstract
SYNOPSIS. Twenty strains of hartmannellid amoebae have been studied. All the strains grew axenically in liquid and chemically defined media. Strains were found to differ their rate of growth, optimum temperature for growth and in amino acid requirements. All except one strain (Lilly) were found to be serologically related by the immobilization test. Direct observation of the trophozoites and cysts, and the results of serological and physiological tests, have led to the conclusion that the 20 strains should comprise a single species until further information is gained on these and other representatives of the genus.Earlier literature on the classification of hartmannellid amoebae has been critically reviewed, and it is concluded that Hartmannella castellanii Douglas 1930 is the correct name for the species. It is suggested that Acanthamoeba castellanii (Volkonsky 1931), Hartmannella glebae (Dobell 1914) and H. rhysodes Singh 1952 became synonyms of H. castellanii, and that Mayorella palestinensis Reich 1933 be transferred to the genus Hartmannella.Some strains of H. castellanii were able to grow at mammalian body temperature. Since the species appears to be common and widely distributed, and farms cysts which resist desiccation, it is probable that the occurrence of amoebae in mammalian cell cultures has arisen by contamination from the air.
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