A comparative study of cellulase production in inorganic and organic supplements by a cellulolytic tree bark fungus

V Vijeetha,A Jayakumaran Nair

doi:10.14719/pst.2020.7.2.708

Abstract

The study was conducted to compare the cellulase production using organic and inorganic supplements by isolating a tree bark (Mangifera indica) fungus, for a cost-effective cellulase production. Three fungi (f1, f2 and f3) were isolated from the bark of the tree, of which f3 was found to be potential in cellulase production which was confirmed by primary screening (congo red activity staining). Through endoglucanase and total cellulase activity assays, it was observed that the isolated strain exhibited cellulase activity of 0.25 U/ml for endoglucanase when 1% CMC was supplemented to the medium. An activity of 8 IU/g towards FPase and for endoglucanase 10.35 U/g was obtained on non-purified inorganic supplements. Sugarcane bagasse was found to be the best inorganic supplement for cellulase production. Here, we try to adopt a cost-effective production strategy of cellulase. The future perspective of this investigation is to identify the strain and purification of the enzyme for industrial purpose.

Highlights

Cellulose is the major constituent of the plant cell wall
Three fungi (f1, f2 and f3) were isolated from the bark of the tree, of which f3 was found to be potential in cellulase production which was confirmed by primary screening
Through endoglucanase and total cellulase activity assays, it was observed that the isolated strain exhibited cellulase activity of 0.25 U/ml for endoglucanase when 1% CMC was supplemented to the medium

Summary

Introduction

Cellulose is the major constituent of the plant cell wall. It is the most abundant organic compound found on earth surface [1, 2]. Plants produce 1.5 x 1012 tons of cellulose annually which is a highly stable crystalline polymer consisting of β-1, 4 –linked glycosyl residues, along other polysaccharides [3]. With this bulk production, its turnover in the carbon cycle is of great importance. The conversion of cellulose to glucose achieved through enzymatic hydrolysis is preferred over chemical methods [4]

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