Abstract
Since [ 3H]thymidine ([ 3H]TdR) and [ 125I]iododeoxyuridine ([ 125I]UdR) appear to label very different cell populations in different tissues, we carried out the present investigation to determine the value and limitations of the use of [ 125I]UdR as a DNA precursor in a study of grafted cell proliferation during the course of a graft-versus-host reaction in mice. Results showed that the iodine radioisotope was as efficient as [ 3H]thymidine when endogenous thymidylate synthetase was inhibited by fluorodeoxyuridine. The use of 125I as an in vivo labelling compound constitutes a simple technique for evaluating DNA synthesis, provided the unfixed labelled iodine is eliminated by in vitro ethanol washing of tissues. The optimal conditions for elution are defined but may vary from one tissue to another. [ 125I]Udr and [ 3H]TdR give similar pictures of the very intricate phenomena accompanying a chronic graft-versus-host reaction (GVHR) induced in mice by minor histoincompatible antigens.
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