Abstract
Objectives: Introduction of solid phase assays for HLA antibodies detection led to application of new algorithms for the immunological monitoring of patients in renal transplantation and prevented many cases of hum oral rejection. Methods: In this work, we made a single observation of a comparative study between ELISA and LUMINEX® on 60 patient’s sera prior to start using in routine LUMINEX® at the two compatibility department of Pasteur Institute Casablanca, Morocco. Data were analyzed following manufacturer recommendations. Finding: Concordance rates between the results for individual HLA Class I and Class II antigens measured by both techniques were found to be 73, 3%. Whereas discrepancies were around 25%. The use of sensitive and specific techniques in transplantation is an important parameter which could improve this field. Improvement: Following this study, significant difference between the tests was found, however LUMINEX detect HLA antibodies with higher sensitivity, which can provide additional information for the graft outcome. Keywords: Anti-HLA Antibodies, Kidney Transplantation, ELISA, LUMINEX
Highlights
Anti Human Leukocyte Antigen / DSA (HLA) antibodies, are formed by the immune system, they occur following multiple pregnancies, blood transfusions, and previous transplantation
We provide an additional data regarding the efficiency of solid phase assays which are an important asset to the clinician in kidney transplantation
While discrepancy was obtained for 16 sera patients (26, 6%), where 10 sera were ELISA negative/LUMINEX positive, and 6 sera ELISA positive- LUMINEX negative. (p = 0.006) (Table 1)
Summary
Anti HLA antibodies, are formed by the immune system, they occur following multiple pregnancies, blood transfusions, and previous transplantation. In the middle of 90’s, the introduction of solid phase assays has allowed a better and more specific detection of antibodies; such as the Enzyme Linked Immunosorbent assay method where antigen are fixed in TERASAKI plate, and the LUMINEX technical where antigen are coated in animusofluorescent beads. The latter technical was recently introduced at the two compatibility unit at Pasteur Institute of morocco, whence the importance to lead a comparative study between both ELISA and LUMINEX for screening of anti HLA antibodies
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