Abstract
This investigation involves development of a new kinetic spectrophotometric and a reverse phase-high performance liquid chromatography (RP-HPLC) methods for the determination of aspartame AS in pharmaceutical preparations. Spectrophotometric method was based on the oxidation of the drug AS with alkaline potassium permanganate. The reaction is followed spectrometrically by measuring the rate change of the absorbance of AS at 600nm. A fixed-time (at 48 min) method is adopted for determining the drug concentration. A linear calibration graph was in the range of 1-7μg.ml-1, with a correlation coefficient of 0.9998, detection limit of 0.101μg.ml-1, molar absorption coefficient is 5.2×104 L/mol.cm, Sandels sensitivity (S) 0.0056 μg/cm2 and relative standard deviation RSD% of 1.40%. In HPLC method, the drug was analyzed using RP-HPLC method with a Zorbax ODS-C18 (15cm×4.6mm i.d); analytical column (5μm partical size) and Isocratic elution with a mobile phase containing 15% acetonitrile in 0.02M sodium acetate buffer (pH 5.4), at a flow rate of 1ml.min-1, 20 μl sample loop, and the UV detector was set at λmax 220nm, Calibration graph was in the range of 10-70 μg.ml-1 with a correlation coefficient of 0.9991, detection limit of 1.09μg.ml-1 and a relative standard deviation of 0.91%.The two methods were applied successfully to determine the content of AS in pharmaceutical preparations with a recovery of 98.8-99.3%.
Highlights
Aspartame: (S)-3-amino-N-[(S)-1-(methoxy carbonyl)-2-phenyl ethyl](British pharmacopoeia,2000) Succinamic acid is a white crystalline powder.The empirical formula for (AS) is C14H18N2O5 and its molecular weight is 294.3, it is used as sweetening agent and its structure is: Many methods have been developed for the determination of Aspartame in various matrices such as pharmaceutical preparation, beverages, saccharin, foods, chocolate, soya-bean products, desserts, sweeteners, soft drinks and in fermented milk beverages
The present paper describe a spectrophotometric method for the determination of aspartame in pharmaceutical preparations based on the oxidation with alkaline potassium permanganate
The various experimental parameters affecting the formation of the reaction product were optimized as follows: Effect of KMnO4 concentration To study the effect of the KMnO4 concentration, aliquots of (AS)
Summary
Aspartame: (S)-3-amino-N-[(S)-1-(methoxy carbonyl)-2-phenyl ethyl](British pharmacopoeia,2000) Succinamic acid is a white crystalline powder.The empirical formula for (AS) is C14H18N2O5 and its molecular weight is 294.3, it is used as sweetening agent and its structure is: Many methods have been developed for the determination of Aspartame in various matrices such as pharmaceutical preparation, beverages, saccharin, foods, chocolate, soya-bean products, desserts, sweeteners, soft drinks and in fermented milk beverages. The present paper describe a spectrophotometric method for the determination of aspartame in pharmaceutical preparations based on the oxidation with alkaline potassium permanganate. The proposed method was applied successfully for the determination of the analyte in pharmaceutical preparations. RP-HPLC method was compared successfully with kinetic spectrophotometry and were applied for the analysis of pharmaceutical preparations samples containing aspartame.
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