A Comparative Study Between Porcine Peritoneum and Pericardium as Cardiovascular Material.

Abstract

Decellularized porcine pericardium has many applications in the cardiovascular field for its excellent properties. The peritoneum is a single-layer bio-dialysis membrane with many similarities and differences in physical characteristics, biochemical composition, and structure to the pericardium. The limited available literature suggests that, similar to the pericardium, the peritoneum has good application potential in the field of cardiovascular substitute materials. This research focused on comparing the differences between decellularized peritoneum and decellularized pericardium in microstructure, biochemical composition, mechanical properties, hemocompatibility, in vitro enzymatic degradation, in vitro calcification, cytocompatibility, and other vital indicators. The peritoneum was consistent with pericardium in terms of fibrous structure, hemocompatibility, in vitro calcification, and cytocompatibility. The peritoneal elastic fiber content (219 μg/mg) was significantly higher than that of the pericardium (66 μg/mg), resulting in two to three times higher maximum load (21.1 N) and burst pressure (1309 mmHg), and better performance than the pericardium in terms of in vitro resistance to enzymatic degradation. In the cardiovascular field, decellularized peritoneum can be used as vascular substitute material. Impact statement There are many similarities between the embryonic origin and morphological structure of the porcine peritoneum and the porcine pericardium, but little research has been done on the use of the porcine peritoneum as a biomaterial. In this compared research, we showed that porcine peritoneum had better resistance to enzymatic degradation, better stretching, and more suitable burst pressure for being used as vascular substitute material. This research is the first to describe the structural composition of porcine peritoneum and its advantageous properties as a cardiovascular material.