Abstract
The presence of higher level of exogenous growth hormone (GH) in transgenic animals could lead to several physiological alterations. A GH transgenic zebrafish (Danio rerio) line was compared to nontransgenic (NT) samples of the species through a DDRT-PCR approach, with the goal of identifying candidate differentially expressed transcripts in brain tissues that could be involved in GH overexpression. Densitometric analyses of two selected amplification products, p300 and ADCY2, pointed to a significant lower gene expression in the transgenic zebrafish (104.02 ± 57.71; 224.10 ± 91.73) when compared to NT samples (249.75 ± 30.08; 342.95 ± 65.19). The present data indicate that p300 and ADCY2 are involved in a regulation system for GH when high circulating levels of this hormone are found in zebrafishes.
Highlights
The growth hormone gene (GH) is primarily responsible for the regulation of processes related to somatic growth in animals
The presence of a higher level of exogenous GH in transgenic animals could lead to metabolic alterations that can influence the animal growth, and several other traits
As the GH secretion in the brain is regulated through a complex neuroendocrine control system that is modulated by hypothalamic peptides and neurotransmitter networks (Müller et al 1991), it would be interesting to determine which genes in this tissue could be associated with GH overexpression
Summary
The growth hormone gene (GH) is primarily responsible for the regulation of processes related to somatic growth in animals. Brain tissue from a transgenic line of Danio rerio (strain F0104) to the growth hormone gene (GH) were used for the identification and characterization of DETs (Differential Expressed Transcripts), through comparisons with brain tissue from a nontransgenic strain of this species.
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