A comparative experimental study to evaluate the anti-epileptic activity of Kalyanaka Ghrita and Ksheera Kalyanaka Ghrita by PTZ induced generalised seizure method

Abstract

Background: One of the major concerns about Epilepsy is the psychological and cognitive effects of commonly used Anti-epileptic drugs. In the treatment aspects related to psychological or psychosomatic diseases, Ayurvedic drug has stood the test of time as they don’t produce any undesirable side effects. Kalyanaka Ghrita is widely used to treat the conditions like Unmada, Apasmara etc. Along with the reference of Kalyanaka Ghrita, Acharya Chakrapanidatta has explained Ksheerakalyanaka Ghrita. They only differ in the ratio of water added as well as the addition of four parts of Ksheera in case of Ksheerakalyanaka Ghrita. The current study aims to evaluate the anti-epileptic activity of Kalyanaka Ghrita and Ksheerakalyanaka Ghrita by Pentylenetetrazole(PTZ) Induced Generalised Seizure method in Swiss Albino Mice. Aims and objectives: To evaluate the antiepileptic activity of Kalyanaka Ghrita and Ksheerakalyanaka Ghrita by PTZ Induced Generalised Seizure Method. Methodology: Group specific drugs were administered for 21 consecutive days by oral route. Diazepam was taken as reference standard drug. On 22nd day, a single dose of Pentylenetetrazole 80mg/ kg body weight was injected intra peritoneal to all the groups. The effect of different formulations on Pentylenetetrazole induced generalised convulsions were noted down. The results were expressed as Mean ± SEM. The data was analysed by one way Anova followed by Dunnet’s multiple ‘t’ test as post HOC using Graph pad Inst 3. Results: There was an increase in the duration of latency of onset of seizures in Kalyanaka ghrta group and Ksheerakalyanaka ghrita group. There was a decrease in the occurrence of number of myoclonic convulsions in Kalyanaka ghrta group and Ksheerakalyanaka group. There was an increase in the number of clonic convulsions in Kalyanaka ghrta group and Ksheerakalyanaka ghrita. There was an increase in the number of straub tail occurrence in Kalyanaka ghrta group and a decrease in the number of straub tail occurrence in Ksheerakalyanaka Ghrta group. The latency of occurrence of death was reduced in Kalyanaka ghrta and Ksheerakalyanaka ghrita group. There was a decrease in the number of recurrent clonic jerks in Kalyanaka ghrta group and an increase in the number of recurrent clonic jerks in Ksheera Kalyanaka ghrta group. Conclusion: Kalyanaka Ghrita and Ksheerakalyanaka Ghrita showed statistically non-significant improvement in the management of symptoms of PTZ Induced Generalised Seizure.