A Comparative Evaluation of Apical Microleakage in Immediate vs Delayed Postspace Preparation using Four Endodontic Sealers seen under Stereomicroscope - An in vitro study

Abstract

Context: An In-vitro research. Introduction: To evaluate the apical microleakage in immediate v/s delayed postspace preparation using four endodontic sealers; Endomethasone N, AH Plus, MTA Fillapex and Gutta Flow. Materials and Methods: One hundred and thirty recently excised single-rooted permanent teeth without caries were decorated. The postspace was created with a Peeso reamer from size 1–4, and 5 mm of gutta-percha was left after the root canals were biomechanically prepared up to F3 (ProTaper file) and obturated using the lateral condensation method. According to immediate and delayed postspace preparation, the samples were separated into eight groups, with 15 samples in each experimental group and 5 samples in each of the two control groups. Except for the apical 2 mm and the negative group, which were entirely sealed with two layers of sticky wax, all of the samples were covered with two layers of oppressive wax. Then, the samples were immersed in 2% methylene blue dye, centrifuged at 3000 rpm for 3 min, sectioned longitudinally and observed the extent of dye penetration under stereomicroscope. To compare the results between study groups and within each group, the Post hoc Tukey’s honestly significant difference comparison test and one-way ANOVA were utilized. When the P value was proved to be less than 0.05, the findings were deemed statistically significant. Results: Between all experimental groups, all samples revealed statistically significant differences. Group V (Endomethasone N-D) 3.46 ± 0.26 showed the greatest microleakage, whereas Group II (AH Plus I) 1.50 ± 0.40 showed little microleakage. Conclusions: Within the constraints of this study, postspace that was promptly prepared with all sealers experienced much-reduced microleakage.