Abstract
Abstract The aim of this work was to determine the antimicrobial activity of chitooligosaccharides (chito) and comparing them with that of the standards broad spectrum antibiotic flomox and kluacid. The chito were prepared by enzyme hydrolysis of chitosan. They were fractionated into four groups (1, 2, 3 and 4) according to their molecular weights (MW) by ultrafiltration. Specific growth rate of the tested microorganisms was determined in the presence of chito. The potential antimicrobial activity of chito was evaluated as minimum inhibitory concentration (MIC) towards Gram positive bacteria (B. cereus), Gram negative fungi (P. aeruginosa) and Gram positive yeast (C. albicans). MIC was determined by8 serial dilution methods. The inhibitory activities of chito 1 and 3 were identified as being as strong as broad spectrum flomox and kluacid against B. Cereus. The specific growth rate of C. albicans was completely inhibited by chito (4) at concentration of 0.11 mg/ml with molecular weight less than 1.0 KDa and was stronger than that of broad spectrum kluacid (0.42 mg/ml). Chito (3) with molecular weight 1–10 KDa had MIC 1.67 mg/ml against P. aeruginosa. The results of this study suggested that chito can be a potent factor affecting as an antimicrobial activity. They could be considered as a possible alternative/additive to known antimicrobial agents in pharmaceutical compositions.
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