Abstract

Aim: This research aims to carry out a comparative assessment on the use of pig manure and poultry manure as a carbon substrate for the degradation of diesel.
 Study Design: Fifteen experimental pots were used in this analysis and this was carried out for a duration of 44 days. This experiment was done in stages and each stage was for 2 weeks. Experimental pots were labelled appropriately with the right concentrations of soil, diesel oil and manure to avoid cross-contamination. This experiment involved one pollution level of 0.275% of the weight of soil. Two (2) treatment concentrations of 20% and 40% of the weight of soil for pig manure and poultry manure respectively, three temperature levels of 25ºC, 30ºC, 37ºC.
 Place and Duration of Study: Microbiological laboratory and Environmental Science laboratory of Coventry University/two months.
 Methodology: Bioremediation of the diesel contaminated soil involved two concentration levels of 40 g and 80 g. These concentrations (40 g and 80 g) were used for both pig manure and poultry manure. These manures were measured in the fume cupboard using an electronic balance to avoid pollution to air. The right manure concentrations of 40 g and 80 g were transferred into the appropriately labelled pots containing 200 g of soil each. The mixture (soil+ diesel+ pig/poultry manure) was properly homogenised and allowed for biodegradation.
 Results: Results of TPH analysis showed high percent removal of 84.71%, 90%, 82.35%, 85.29% for soil treated with pig manure at 40 g and 80 g, and soil treated with 40 g, 80 g of poultry manure at 37ºC. This study showed that the microbial consortium, nutrient concentration and temperatures played great roles in enhancing bioremediation process.
 Conclusion: Nutrient addition enhanced the degradation of diesel contaminated soil. It is evident from the results that at 37ºC diesel degradation occurred more in all soil samples than at 30oC and 25ºC. Therefore, it can be concluded that 37ºC is most suitable for diesel degradation with the highest efficiency in soil treated with 80 g of pig manure. However, at 25oC, high percent degradation also occurred in all treated samples with spi-40 g having the highest percent degradation.

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