A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk Fibroin.

Liubov Safonova,Igor Agapov,Maria Bobrova,Anton Efimov,Alexey Lyundup,Olga Agapova

doi:10.3390/pharmaceutics13101561

Liubov Safonova, Igor Agapov + Show 4 more

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https://doi.org/10.3390/pharmaceutics13101561

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Journal: Pharmaceutics	Publication Date: Sep 26, 2021
Citations: 4	License type: CC BY 4.0

Affiliation: Peoples' Friendship University of Russia

Abstract

A comparative analysis of the structure and biological properties of silk fibroin constructions was performed. Three groups of constructions were obtained: films obtained by casting an aqueous solution of silk fibroin and electrospun microfibrous scaffolds based on silk fibroin, with the addition of 30% gelatin per total protein weight. The internal structures of the films and single fibers of the microfibrous scaffolds consisted of densely packed globule structures; the surface area to volume ratios and volume porosities of the microfibrous scaffolds were calculated. All constructions were non-toxic for cells and provide high levels of adhesion and proliferation. The high regenerative potential of the constructions was demonstrated in a rat full-thickness skin wound healing model. The constructions accelerated healing by an average of 15 days and can be considered to be promising constructions for various tasks of tissue engineering and regenerative medicine.

Highlights

The development of biocompatible materials is one of the priorities of tissue engineering
In the course of the research, constructions with various micro- and nano-structures were obtained: films based on silk fibroin and microfibrous scaffolds based on silk fibroin and gelatin
The surface of the obtained films has a relief in the form of micro- and nano-roughness, and their internal structure is characterized by the presence of silk fibroin globules and the absence of pores

Summary

Introduction

The development of biocompatible materials is one of the priorities of tissue engineering. Natural materials with biocompatibility and biodegradability without the formation of toxic degradation products are preferred [6,7,8]. Components of the native extracellular matrix, such as collagen, fibronectin, elastin, hyaluronic acid, etc., and polymers with a natural origin (alginates, chitosan, etc.) are widely used, as well as composites based on these materials [9,10]. One of these materials is gelatin, which is used as a composite additive to improve biocompatibility through its solubility and ability to utilize many novel methods, such as electrospinning [11]

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