A comparative analysis of antioxidant system and bioremediation of chromium in Pseudomonas sp. Cr13 and its transgenic strain F1251

Abstract

The present study describes the Cr(VI) bioaccumulation potential of Pseudomonas sp. Cr13 and its transgenic strain F1251. Compared with strain Cr13, transgenic strain F1251 with the antisense CadR gene encoding Cd(II)/Pb(II)-responsive transcriptional regulator (GenBank Accession no., OP564917) showed the higher removal efficiency of Cr(VI), the enhanced activities of antioxidative enzymes such as SOD (superoxide dismutase), GR (glutathione reductase), CAT (catalase), APX (ascorbate peroxidase), POD (peroxidase), the increased exopolysaccharide content and the decreased malondialdehyde content. The transgenic strain F1251 could more efficiently bioaccumulate Cr(VI) compared with strain Cr13, which provided a better solution for the removal of metal pollutant Cr(VI). • Cr removal of Pseudomonas sp. Cr13 and its transgenic strain F1251 are evaluated. • The significant difference in antioxidant system was found between Cr13 and F1251. • Malondialdehyde and exopolysaccharide contents was compared between Cr13 and F1251. • Strain F1251 could more efficiently bioaccumulate Cr(VI) compared with strain Cr13.