Abstract
A highly sensitive, rapid immunoassay performed in the multi-channels of a micro-well array consisting of a multicapillary glass plate (MCP) and a polydimethylsiloxane (PDMS) slide is described. The micro-dimensions and large surface area of the MCP permitted the diffusion distance to be decreased and the reaction efficiency to be increased. To confirm the concept of the method, human immunoglobulin A (h-IgA) was measured using both the proposed immunoassay system and the traditional 96-well plate method. The proposed method resulted in a 1/5-fold decrease of immunoassay time, and a 1/56-fold cut in reagent consumption with a 0.05 ng/mL of limit of detection (LOD) for IgA. The method was also applied to saliva samples obtained from healthy volunteers. The results correlated well to those obtained by the 96-well plate method. The method has the potential for use in disease diagnostic or on-site immunoassays.
Highlights
The development of simple, rapid immunoassay approaches is of tremendous importance for on-site diagnostics and for monitoring highly infectious diseases [1]
We previously reported on the use of multicapillary glass plate (MCP) combined with a microbead-based chemiluminescence immunoassay [19], in which it acted as a switch for bonding/free protein separation
A PDMS slide was prepared by mixing the PDMS prepolymer and curing crosslinker at a mass ratio of 18:1 and the resulting mixture was degassed in vacuo
Summary
The development of simple, rapid immunoassay approaches is of tremendous importance for on-site diagnostics and for monitoring highly infectious diseases [1]. Infectious diseases, such as the avian influenza virus, severe acute respiratory syndrome (SARS), hand-foot-and-mouth disease, influenza. A MCP with uniformly paralleled microchannels would be an ideal candidate for use in an immunoassay. Such a material has been used for DNA hybridization reactions [18], in which MCP showed substantial advantages over flat glass, in terms of its higher sensitivity and a faster hybridization rates. The compact immunoassay system shows considerable potential in terms of speed and high-sensitivity for on-site diagnosis and for rapidly monitoring a disease
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
