Abstract
Lactacystin, a selective inhibitor of some proteasome activities, in the presence of 12-tetradecanoyl phorbol-13-acetate stimulates the release of arachidonic acid from the phospholipids of bovine pulmonary artery endothelial, bovine embryonic thoracic aorta smooth muscle, rat liver and rat glial cells. The enhanced release from rat liver cells is inhibited by dexamethasone, aspirin, Na salicylate and the immunosuppressive fungal metabolite, gliotoxin. The effects of aspirin plus dexamethasone are not additive, but those of gliotoxin plus dexamethasone are additive. The stimulated and unstimulated release from all four cells are decreased when these cells are grown to confluence or when they are growth arrested by serum deprivation. The unstimulated as well as the stimulated release is partially inhibited by the cytosolic phospholipase A2inhibitor, methylarachidonyl fluorophosphonate. The enhanced release is reduced in the presence of the translation and transcription inhibitors, actinomycin and cycloheximide.
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