Abstract
Gold nanoparticles (AuNPs) are used for different applications such as catalysis and biosensing. For numerous uses related to biosensing and therapy, AuNPs functionalization by thiols is required. The thiolation of AuNPs has been widely studied in nanoparticles synthesized by chemical approaches. However, studies of the thiolation of bare nanoparticles that do not include citrate-metal surfaces are scarce. Here, we propose a methodology to evaluate the thiolation process and its effectiveness on bare AuNPs synthesized by laser ablation in liquids (LAL). The methodology is based on an Ellman's assay that is easy to implement. UV–vis-NIR spectroscopy, TEM imaging, DLS, and colloidal stability of the bare AuNPs due to the thiolation are also presented.
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