Abstract
Because of the toxicity of hydrogen sulfide and its important role in various physiological processes, the development of a selective and sensitive hydrogen sulfide sensing method is vital. In this study, a colorimetric method for hydrogen sulfide detection was developed by exploiting the direct inhibition of active site in G-quadruplex DNAzyme by hydrogen sulfide. Hydrogen sulfide inactivates the peroxidase-like activity of PS2.M-hemin G-quadruplex DNAzyme by directly blocking the active site of the DNAzyme, allowing the label-free colorimetric detection of hydrogen sulfide by the naked eye by using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS2−) as a peroxidase substrate. From titration results, the detection limit of this colorimetric hydrogen sulfide sensor was estimated to be 410 nM. In addition, the sensor is highly selective for hydrogen sulfide over various anions and was used to determine hydrogen sulfide concentrations in tap water samples.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
